nm4bl2108 – Hybrid multifunctional bioinks for 3D printed disease models

NM4BL-2021-Poster-Uxue-Aizarna
Hybrid multifunctional bioinks for 3D printed disease models
Uxue Aizarna Lopetegui1, Luis Liz-Marzán1,2,3, Malou Henriksen-Lacey1,2, Dorleta Jimenez
de Aberasturi1,2,3
1 CIC biomaGUNE, Basque Research and Technology Alliance (BRTA), Paseo de Miramón 182,
Donostia-San Sebastián 20014, Spain. 2Ciber-BBN, 20014 Donostia-San Sebastián, Spain;
3Ikerbasque, Basque Foundation for Science, 48013 Bilbao, Spain

3D printing and nanotechnology have emerged as promising tools that contribute to advancing
biomedical research, including the fabrication of complex in vitro tissue models that can give us
further insight into cancer progression1,2. Organic-inorganic hybrid materials, composed of different
polymers or hydrogel compositions that include inorganic nanoparticles (NPs) and living cells, can
be used to produce the composite bioinks for 3D printed models3,4. We are studying the effect of
combining different material layers which have stimuli-responsive functionality to use these bioinks
for the fabrication of realistic disease models by use of 3D printing. Thermal, structural, optical and
rheological characterization methods are employed to select the ideal materials to represent the
physiological and pathophysiological conditions. The results obtained so far suggest the suitability
of using 3D printing technology for the generation of 3D tissue models based on smart-hybrid living
bioinks.

Figure 1. (A) Structural characterization by SEM imaging of 3D-printed cell containing layer
composed of 5% GelMA – 1% alginate gel. (B) 3D printed agarose cylinder containing AuNRs. (C)
3D printed GelMA –alginate hydrogel containing living HDF – GFP cells showing cell spreading after
14 days post-printing, highlighting the biocompatible nature of the bioink. (D) TEM image of AuNRs.
Keywords: 3D printing, smart-hybrid materials, living bioinks.
References
[1] P. A. Mollica, Acta Biomaterialia, 2019, 95, 201-213.
[2] Y. C. Oztan, Bioprinting, 2020, e00079.
[3] A. Bhattacharyya, Addit. Manuf., 2021, 37.
[4] K. Zhu, Adv. Funct. Mater., 2018, 27, 12.